Journal: Journal of anatomy

Article Title: Expression of Toll-like receptor 9 in mouse and human lungs.

doi: 10.1111/joa.12039

Figure Lengend Snippet: Fig. 1 TLR9 in situ hybridization on mouse lungs. Lung sections stained using TLR9 DIG-labeled RNA show staining (arrows) in several cell types (A). Lung sections from mice stained without a specific probe (B) lacks staining in any tissue. High magnification shows staining in vascular endo- thelium (arrowhead) (C), septal (arrow) as well as alveolar macrophages (chevron) (D), and bronchial epithelium (double arrow) (E). Scale bar: 100 lm.

Article Snippet: Immuno-electron microscopy was carried out as described previously (Schneberger et al., 2009) with TLR9 antibody (1 : 50) and 20 nm gold-conjugated anti-mouse secondary antibody (1 : 100; Fitzgerald Industries International, Concord, MA, USA) for 1 h. The controls included protocols without the primary antibody and staining of sections with vWF antibody.

Techniques: In Situ Hybridization, Staining, Labeling

Journal: Journal of anatomy

Article Title: Expression of Toll-like receptor 9 in mouse and human lungs.

doi: 10.1111/joa.12039

Figure Lengend Snippet: Fig. 2 TLR9 immunohistochemistry on mouse lungs. Lung sections from mice stained with only a secondary antibody (A) lack staining in alveolar septa, whereas those stained with vWF antibody (B) shows staining in endothelium (arrowhead) alone. Lung sections stained using TLR9 antibody show staining in several tissues (arrows) (C), including bronchial epithelium (double arrow) (D), vascular endothelium (arrowhead) (E), and septal (arrow) as well as alveolar macrophages (chevron) (F). Scale bar: 100 lm.

Article Snippet: Immuno-electron microscopy was carried out as described previously (Schneberger et al., 2009) with TLR9 antibody (1 : 50) and 20 nm gold-conjugated anti-mouse secondary antibody (1 : 100; Fitzgerald Industries International, Concord, MA, USA) for 1 h. The controls included protocols without the primary antibody and staining of sections with vWF antibody.

Techniques: Immunohistochemistry, Staining

Journal: Journal of anatomy

Article Title: Expression of Toll-like receptor 9 in mouse and human lungs.

doi: 10.1111/joa.12039

Figure Lengend Snippet: Fig. 3 TLR9 staining in a mouse neutrophil. TLR9 staining (arrows) observed in mouse lung neutrophil, epithelium (arrowhead), and endothelium (chevron). N, nucleus; AS, alveolar space. Scale bar: 4 lm.

Article Snippet: Immuno-electron microscopy was carried out as described previously (Schneberger et al., 2009) with TLR9 antibody (1 : 50) and 20 nm gold-conjugated anti-mouse secondary antibody (1 : 100; Fitzgerald Industries International, Concord, MA, USA) for 1 h. The controls included protocols without the primary antibody and staining of sections with vWF antibody.

Techniques: Staining

Journal: Journal of anatomy

Article Title: Expression of Toll-like receptor 9 in mouse and human lungs.

doi: 10.1111/joa.12039

Figure Lengend Snippet: Fig. 4 TLR9 staining in a mouse type-II cell. TLR9 staining (arrows) observed in a mouse lung type-II cell. N, nucleus; AS, alveolar space; LB, lamellar bodies. Scale bar: 4 lm.

Article Snippet: Immuno-electron microscopy was carried out as described previously (Schneberger et al., 2009) with TLR9 antibody (1 : 50) and 20 nm gold-conjugated anti-mouse secondary antibody (1 : 100; Fitzgerald Industries International, Concord, MA, USA) for 1 h. The controls included protocols without the primary antibody and staining of sections with vWF antibody.

Techniques: Staining

Journal: Journal of anatomy

Article Title: Expression of Toll-like receptor 9 in mouse and human lungs.

doi: 10.1111/joa.12039

Figure Lengend Snippet: Fig. 6 Human lung TLR9 in situ RNA hybridization. Lung sections from normal (A) or COPD (B) patients stained using TLR9 DIG-labeled RNA probe show staining (arrows) in several cell types with a clear influx of TLR9 positive cells in the bronchus. Staining without a RNA probe shows lack of staining in any tissue (C). High magnification shows staining in bronchial epithelium (double arrow) (E), vascular endothelium (arrowhead) (F), and septal (arrow) as well as alveolar (chevron) macrophages (G). Scale bar: 100 lm.

Article Snippet: Immuno-electron microscopy was carried out as described previously (Schneberger et al., 2009) with TLR9 antibody (1 : 50) and 20 nm gold-conjugated anti-mouse secondary antibody (1 : 100; Fitzgerald Industries International, Concord, MA, USA) for 1 h. The controls included protocols without the primary antibody and staining of sections with vWF antibody.

Techniques: In Situ, Hybridization, Staining, Labeling

Journal: Journal of anatomy

Article Title: Expression of Toll-like receptor 9 in mouse and human lungs.

doi: 10.1111/joa.12039

Figure Lengend Snippet: Fig. 5 TLR9 staining in a mouse alveolar macrophage cell. TLR9 staining (arrows) observed in a mouse alveolar macrophage. N, nucleus; AS, alveolar space. Scale bar: 4 lm.

Article Snippet: Immuno-electron microscopy was carried out as described previously (Schneberger et al., 2009) with TLR9 antibody (1 : 50) and 20 nm gold-conjugated anti-mouse secondary antibody (1 : 100; Fitzgerald Industries International, Concord, MA, USA) for 1 h. The controls included protocols without the primary antibody and staining of sections with vWF antibody.

Techniques: Staining

Journal: Journal of anatomy

Article Title: Expression of Toll-like receptor 9 in mouse and human lungs.

doi: 10.1111/joa.12039

Figure Lengend Snippet: Fig. 7 Human lung TLR9 immunohistochemistry. Lung sections from human patients stained with only a secondary antibody (A) lack staining in alveolar septa, whereas those stained with vWF antibody (B) show staining in endothelium (arrowhead) alone. Lung sections stained using TLR9 antibody show staining (arrows) in several cell types in both normal (C) as well as COPD patients (D). High magnification shows staining in bron- chial epithelium (double arrow) (E), vascular endothelium (arrowhead) (F), and septal (G) as well as alveolar macrophages (chevron) (H). Scale bar: 100 lm. Field counts of control and COPD patients showed a greater density of TLR9 positive cells in those with COPD (P < 0.01).

Article Snippet: Immuno-electron microscopy was carried out as described previously (Schneberger et al., 2009) with TLR9 antibody (1 : 50) and 20 nm gold-conjugated anti-mouse secondary antibody (1 : 100; Fitzgerald Industries International, Concord, MA, USA) for 1 h. The controls included protocols without the primary antibody and staining of sections with vWF antibody.

Techniques: Immunohistochemistry, Staining, Control

Journal: Journal of anatomy

Article Title: Expression of Toll-like receptor 9 in mouse and human lungs.

doi: 10.1111/joa.12039

Figure Lengend Snippet: Fig. 8 TLR9 staining in human alveolar macrophage. TLR9 staining (arrows) observed in a human alveolar macrophage. N, nucleus; AS, alveolar space. Scale bar: 4 lm.

Article Snippet: Immuno-electron microscopy was carried out as described previously (Schneberger et al., 2009) with TLR9 antibody (1 : 50) and 20 nm gold-conjugated anti-mouse secondary antibody (1 : 100; Fitzgerald Industries International, Concord, MA, USA) for 1 h. The controls included protocols without the primary antibody and staining of sections with vWF antibody.

Techniques: Staining

Journal: Journal of anatomy

Article Title: Expression of Toll-like receptor 9 in mouse and human lungs.

doi: 10.1111/joa.12039

Figure Lengend Snippet: Fig. 9 TLR9 staining in a human type-II cell. TLR9 staining (arrows) observed in a mouse lung type-II cell. N, nucleus; AS, alveolar space. Scale bar: 4 lm.

Article Snippet: Immuno-electron microscopy was carried out as described previously (Schneberger et al., 2009) with TLR9 antibody (1 : 50) and 20 nm gold-conjugated anti-mouse secondary antibody (1 : 100; Fitzgerald Industries International, Concord, MA, USA) for 1 h. The controls included protocols without the primary antibody and staining of sections with vWF antibody.

Techniques: Staining